Theragnostic Technologies mab 7f5

Red fluorescence <t>(7F5@Au/Fe3O4rpar;</t> observed in the membrane of the 7F5@Au/Fe3O4+ PC-3 cells (top row) while no red fluorescence was observed in the membrane of the 7F5@Au/Fe3O4+ SMMC-7721 cells (bottom row). Cell nuclei were stained blue in color via DAPI (middle column). 7F5@Au/Fe3O4 fluorescence images and DAPI images are merged in right-most column. Scale bar, 10 µm.

Mab 7f5, supplied by Theragnostic Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mab 7f5/product/Theragnostic Technologies
Average 90 stars, based on 1 article reviews

mab 7f5 - by Bioz Stars, 2026-03

90/100 stars

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anti-psca mab 7f5 (Macrocyclics Inc)

Macrocyclics Inc anti-psca mab 7f5
$Characterization of purified <t>anti-PSCA</t> mAb <t>7F5.</t> ( A ) The dialyzed elution fraction of the isolated anti-PSCA mAb 7F5 was separated by SDS-PAGE and either subsequently stained with Coomassie Brilliant Blue G250 ( AI ) or followed by immunoblotting ( AII ). SDS-PAGE was performed under non-reducing (( AI ), left lane) or reducing conditions (( AI ), right lane; ( AII )). After transfer to nitrocellulose membrane ( AII ), the presence of IgG heavy and light chains in the purified anti-PSCA 7F5 mAb fraction was verified using anti-mouse IgG conjugated with alkaline phosphatase ( AII ). M, molecular weight marker. ( B ) To test for specific binding of the anti-PSCA mAb 7F5, FACS analyses were performed to either PSCA-positive PC 3 cells (( B ), PC3-P, left panel, grey graph) or PSCA-negative PC3 cells (( B ), PC3, right panel, grey graph). Binding was detected via a phycoerythrin-conjugated goat F(ab’)2 fragment anti-mouse IgG (Fcg). According to previous studies , PC3 cells express the epidermal growth factor receptor only at very low level. As negative control, we therefore stained the cells with an anti-EGFR mAb and the phycoerythrin-conjugated goat F(ab’)2 fragment anti-mouse IgG (Fcg) (( B ), white graphs). Bars represent the percentage of antigen-positive cells. ( C ) Representative specific binding of the anti-PSCA mAb 7F5 to PC3-P cells yielded a K D value of 10.5 ± 4.0 nM (n = 3).$
Anti Psca Mab 7f5, supplied by Macrocyclics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-psca mab 7f5/product/Macrocyclics Inc
Average 90 stars, based on 1 article reviews

anti-psca mab 7f5 - by Bioz Stars, 2026-03

90/100 stars

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anti-psca mabs (mab 7f5) goldmag nanoparticles (GoldMag Nanobiotech)

GoldMag Nanobiotech anti-psca mabs (mab 7f5) goldmag nanoparticles
$Characterization of purified <t>anti-PSCA</t> mAb <t>7F5.</t> ( A ) The dialyzed elution fraction of the isolated anti-PSCA mAb 7F5 was separated by SDS-PAGE and either subsequently stained with Coomassie Brilliant Blue G250 ( AI ) or followed by immunoblotting ( AII ). SDS-PAGE was performed under non-reducing (( AI ), left lane) or reducing conditions (( AI ), right lane; ( AII )). After transfer to nitrocellulose membrane ( AII ), the presence of IgG heavy and light chains in the purified anti-PSCA 7F5 mAb fraction was verified using anti-mouse IgG conjugated with alkaline phosphatase ( AII ). M, molecular weight marker. ( B ) To test for specific binding of the anti-PSCA mAb 7F5, FACS analyses were performed to either PSCA-positive PC 3 cells (( B ), PC3-P, left panel, grey graph) or PSCA-negative PC3 cells (( B ), PC3, right panel, grey graph). Binding was detected via a phycoerythrin-conjugated goat F(ab’)2 fragment anti-mouse IgG (Fcg). According to previous studies , PC3 cells express the epidermal growth factor receptor only at very low level. As negative control, we therefore stained the cells with an anti-EGFR mAb and the phycoerythrin-conjugated goat F(ab’)2 fragment anti-mouse IgG (Fcg) (( B ), white graphs). Bars represent the percentage of antigen-positive cells. ( C ) Representative specific binding of the anti-PSCA mAb 7F5 to PC3-P cells yielded a K D value of 10.5 ± 4.0 nM (n = 3).$
Anti Psca Mabs (Mab 7f5) Goldmag Nanoparticles, supplied by GoldMag Nanobiotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-psca mabs (mab 7f5) goldmag nanoparticles/product/GoldMag Nanobiotech
Average 90 stars, based on 1 article reviews

anti-psca mabs (mab 7f5) goldmag nanoparticles - by Bioz Stars, 2026-03

90/100 stars

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Image Search Results

Red fluorescence (7F5@Au/Fe3O4rpar; observed in the membrane of the 7F5@Au/Fe3O4+ PC-3 cells (top row) while no red fluorescence was observed in the membrane of the 7F5@Au/Fe3O4+ SMMC-7721 cells (bottom row). Cell nuclei were stained blue in color via DAPI (middle column). 7F5@Au/Fe3O4 fluorescence images and DAPI images are merged in right-most column. Scale bar, 10 µm.

Journal: PLoS ONE

Article Title: MRl of Prostate Cancer Antigen Expression for Diagnosis and lmmunotherapy

doi: 10.1371/journal.pone.0038350

Figure Lengend Snippet: Red fluorescence (7F5@Au/Fe3O4rpar; observed in the membrane of the 7F5@Au/Fe3O4+ PC-3 cells (top row) while no red fluorescence was observed in the membrane of the 7F5@Au/Fe3O4+ SMMC-7721 cells (bottom row). Cell nuclei were stained blue in color via DAPI (middle column). 7F5@Au/Fe3O4 fluorescence images and DAPI images are merged in right-most column. Scale bar, 10 µm.

Article Snippet: In this study, mAb 7F5 was conjugated to Au/Fe 3 O 4 nanoparticles to produce novel MRI theragnostic probe ( 7F5@Au/Fe3O4 ) for PCa.

Techniques: Fluorescence, Membrane, Staining

Tube I: PC-3+7F5@Au/Fe3O4 cell sample, tube II: PC-3+ IgG@Au/Fe3O4 sample; tube III: SMMC-7721+7F5@Au/Fe3O4 sample; Tube IV: SMMC-7721+ IgG@Au/Fe3O4 sample. Quantitative T2w SNR measurements ( B ) for the cell sample vials depicted in ( A ).

Journal: PLoS ONE

Article Title: MRl of Prostate Cancer Antigen Expression for Diagnosis and lmmunotherapy

doi: 10.1371/journal.pone.0038350

Figure Lengend Snippet: Tube I: PC-3+7F5@Au/Fe3O4 cell sample, tube II: PC-3+ IgG@Au/Fe3O4 sample; tube III: SMMC-7721+7F5@Au/Fe3O4 sample; Tube IV: SMMC-7721+ IgG@Au/Fe3O4 sample. Quantitative T2w SNR measurements ( B ) for the cell sample vials depicted in ( A ).

Article Snippet: In this study, mAb 7F5 was conjugated to Au/Fe 3 O 4 nanoparticles to produce novel MRI theragnostic probe ( 7F5@Au/Fe3O4 ) for PCa.

Techniques:

PC-3 tumor-bearing mice administered 7F5@Au/Fe3O4 nanoparticles demonstrated marked decreases in T2w tumor signal intensity 6, 12, and 24 hrs post-infusion (top row). No clearly appreciable tumor signal changes were observed for SMMC -7721 tumor-bearing mice at any of the three post-infusion time points (bottom row). Prussian blue staining (PB) showed that Au/Fe3O4 nanoparticles depicted as punctate blue-stained foci in the PC-3 tumor tissues; these deposits were not observed within SMMC-7721 tumor tissues. Scale bars, 10 mm for MRI and 50 µm for Prussian blue staining image.

Journal: PLoS ONE

Article Title: MRl of Prostate Cancer Antigen Expression for Diagnosis and lmmunotherapy

doi: 10.1371/journal.pone.0038350

Figure Lengend Snippet: PC-3 tumor-bearing mice administered 7F5@Au/Fe3O4 nanoparticles demonstrated marked decreases in T2w tumor signal intensity 6, 12, and 24 hrs post-infusion (top row). No clearly appreciable tumor signal changes were observed for SMMC -7721 tumor-bearing mice at any of the three post-infusion time points (bottom row). Prussian blue staining (PB) showed that Au/Fe3O4 nanoparticles depicted as punctate blue-stained foci in the PC-3 tumor tissues; these deposits were not observed within SMMC-7721 tumor tissues. Scale bars, 10 mm for MRI and 50 µm for Prussian blue staining image.

Article Snippet: In this study, mAb 7F5 was conjugated to Au/Fe 3 O 4 nanoparticles to produce novel MRI theragnostic probe ( 7F5@Au/Fe3O4 ) for PCa.

Techniques: Staining

For PC-3 mice, tumor volumes were significantly smaller for 7F5@Au/Fe3O4 treated animals (p<0.05) compared to those mice with IgG@Au/Fe3O4 control probe ( A ). No significant difference in tumor volume progression was observed for SMMC-7721 mice following injection of either 7F5@Au/Fe3O4 or IgG@Au/Fe3O4 probes ( B ).

Journal: PLoS ONE

Article Title: MRl of Prostate Cancer Antigen Expression for Diagnosis and lmmunotherapy

doi: 10.1371/journal.pone.0038350

Figure Lengend Snippet: For PC-3 mice, tumor volumes were significantly smaller for 7F5@Au/Fe3O4 treated animals (p<0.05) compared to those mice with IgG@Au/Fe3O4 control probe ( A ). No significant difference in tumor volume progression was observed for SMMC-7721 mice following injection of either 7F5@Au/Fe3O4 or IgG@Au/Fe3O4 probes ( B ).

Article Snippet: In this study, mAb 7F5 was conjugated to Au/Fe 3 O 4 nanoparticles to produce novel MRI theragnostic probe ( 7F5@Au/Fe3O4 ) for PCa.

Techniques: Control, Injection

$Characterization of purified anti-PSCA mAb 7F5. ( A ) The dialyzed elution fraction of the isolated anti-PSCA mAb 7F5 was separated by SDS-PAGE and either subsequently stained with Coomassie Brilliant Blue G250 ( AI ) or followed by immunoblotting ( AII ). SDS-PAGE was performed under non-reducing (( AI ), left lane) or reducing conditions (( AI ), right lane; ( AII )). After transfer to nitrocellulose membrane ( AII ), the presence of IgG heavy and light chains in the purified anti-PSCA 7F5 mAb fraction was verified using anti-mouse IgG conjugated with alkaline phosphatase ( AII ). M, molecular weight marker. ( B ) To test for specific binding of the anti-PSCA mAb 7F5, FACS analyses were performed to either PSCA-positive PC 3 cells (( B ), PC3-P, left panel, grey graph) or PSCA-negative PC3 cells (( B ), PC3, right panel, grey graph). Binding was detected via a phycoerythrin-conjugated goat F(ab’)2 fragment anti-mouse IgG (Fcg). According to previous studies , PC3 cells express the epidermal growth factor receptor only at very low level. As negative control, we therefore stained the cells with an anti-EGFR mAb and the phycoerythrin-conjugated goat F(ab’)2 fragment anti-mouse IgG (Fcg) (( B ), white graphs). Bars represent the percentage of antigen-positive cells. ( C ) Representative specific binding of the anti-PSCA mAb 7F5 to PC3-P cells yielded a K D value of 10.5 ± 4.0 nM (n = 3).$

Journal: International Journal of Molecular Sciences

Article Title: Preclinical Characterization of the 177 Lu-Labeled Prostate Stem Cell Antigen (PSCA)-Specific Monoclonal Antibody 7F5

doi: 10.3390/ijms24119420

Figure Lengend Snippet: Characterization of purified anti-PSCA mAb 7F5. ( A ) The dialyzed elution fraction of the isolated anti-PSCA mAb 7F5 was separated by SDS-PAGE and either subsequently stained with Coomassie Brilliant Blue G250 ( AI ) or followed by immunoblotting ( AII ). SDS-PAGE was performed under non-reducing (( AI ), left lane) or reducing conditions (( AI ), right lane; ( AII )). After transfer to nitrocellulose membrane ( AII ), the presence of IgG heavy and light chains in the purified anti-PSCA 7F5 mAb fraction was verified using anti-mouse IgG conjugated with alkaline phosphatase ( AII ). M, molecular weight marker. ( B ) To test for specific binding of the anti-PSCA mAb 7F5, FACS analyses were performed to either PSCA-positive PC 3 cells (( B ), PC3-P, left panel, grey graph) or PSCA-negative PC3 cells (( B ), PC3, right panel, grey graph). Binding was detected via a phycoerythrin-conjugated goat F(ab’)2 fragment anti-mouse IgG (Fcg). According to previous studies , PC3 cells express the epidermal growth factor receptor only at very low level. As negative control, we therefore stained the cells with an anti-EGFR mAb and the phycoerythrin-conjugated goat F(ab’)2 fragment anti-mouse IgG (Fcg) (( B ), white graphs). Bars represent the percentage of antigen-positive cells. ( C ) Representative specific binding of the anti-PSCA mAb 7F5 to PC3-P cells yielded a K D value of 10.5 ± 4.0 nM (n = 3).

Article Snippet: The anti-PSCA mAb 7F5 was modified with p-SCN-Bn-CHX-A″-DTPA (Macrocyclics, Plano, TX, USA) by coupling the functional isothiocyanate group to primary amines in the protein structure.

Techniques: Purification, Isolation, SDS Page, Staining, Western Blot, Membrane, Molecular Weight, Marker, Binding Assay, Negative Control

Influence of anti-PSCA mAb 7F5 on the viability of PC3-P or PC3 cells measured by the MTS proliferation test as described under after different incubation times (4 h (black), 24 h (gray) and 48 h (white) of treatment); (n = 3). The results were normalized to the untreated medium control and are shown as percentage of living cells; values are averages ± SD from 3 to 4 experiments. As cytotoxic positive control served a treatment with Triton X-100 (2.5%) performed at 4 h (n = 12).

Journal: International Journal of Molecular Sciences

Article Title: Preclinical Characterization of the 177 Lu-Labeled Prostate Stem Cell Antigen (PSCA)-Specific Monoclonal Antibody 7F5

doi: 10.3390/ijms24119420

Figure Lengend Snippet: Influence of anti-PSCA mAb 7F5 on the viability of PC3-P or PC3 cells measured by the MTS proliferation test as described under after different incubation times (4 h (black), 24 h (gray) and 48 h (white) of treatment); (n = 3). The results were normalized to the untreated medium control and are shown as percentage of living cells; values are averages ± SD from 3 to 4 experiments. As cytotoxic positive control served a treatment with Triton X-100 (2.5%) performed at 4 h (n = 12).

Techniques: Incubation, Control, Positive Control

MALDI-ToF and SEC-HPLC analysis of CHX-A″-DTPA-functionalized and non-functionalized anti-PSCA mAb 7F5. ( A ) MALDI-ToF mass spectra of the anti-PSCA mAb 7F5 before [M + H]+ = 150,544 and after conjugation with CHX-A″-DTPA [M + H]+ = 153,578. ( B ) HPLC-SEC elution profiles (UV = 280 nm) of non-conjugated and conjugated anti-PSCA mAb 7F5. Separation was performed using an Agilent Bio SEC-3 column as described under (150 Å, 3 µm, 7.8 ID × 150 mm, 0.1 M sodium phosphate buffer pH 7.0, flow 1 mL/min, 23 °C).

Journal: International Journal of Molecular Sciences

Article Title: Preclinical Characterization of the 177 Lu-Labeled Prostate Stem Cell Antigen (PSCA)-Specific Monoclonal Antibody 7F5

doi: 10.3390/ijms24119420

Figure Lengend Snippet: MALDI-ToF and SEC-HPLC analysis of CHX-A″-DTPA-functionalized and non-functionalized anti-PSCA mAb 7F5. ( A ) MALDI-ToF mass spectra of the anti-PSCA mAb 7F5 before [M + H]+ = 150,544 and after conjugation with CHX-A″-DTPA [M + H]+ = 153,578. ( B ) HPLC-SEC elution profiles (UV = 280 nm) of non-conjugated and conjugated anti-PSCA mAb 7F5. Separation was performed using an Agilent Bio SEC-3 column as described under (150 Å, 3 µm, 7.8 ID × 150 mm, 0.1 M sodium phosphate buffer pH 7.0, flow 1 mL/min, 23 °C).

Techniques: Conjugation Assay

Quality control after 177 Lu-labeling of 7F5-(CHX-A″-DTPA) 4 . ( A ) For estimation of the radiochemical purity, radio-SEC-HPLC profiles were determined prior and after spin filtration in comparison to [ 177 Lu]Lu-CHX-A″-DTPA using a TSKgel SuperSW mAb HR column as described under . ( B ) IRF of [ 177 Lu]Lu-CHX-A″-DTPA-7F5. The radiolabeled Ab was incubated with increasing amounts of PC3-P cells at 37 °C for 2 h. Afterwards, the radiolabeled cells were isolated by filtration through a glass fiber filter. The radioactivity bound to the filter was counted in a gamma counter. Totally applied activity was divided by the specifically bound radioactivity on the cells and then plotted as a function of the reciprocal antigen concentration. The Y intercept represents the reciprocal of the IRF (n = 3, value in % ± SEM).

Journal: International Journal of Molecular Sciences

Article Title: Preclinical Characterization of the 177 Lu-Labeled Prostate Stem Cell Antigen (PSCA)-Specific Monoclonal Antibody 7F5

doi: 10.3390/ijms24119420

Figure Lengend Snippet: Quality control after 177 Lu-labeling of 7F5-(CHX-A″-DTPA) 4 . ( A ) For estimation of the radiochemical purity, radio-SEC-HPLC profiles were determined prior and after spin filtration in comparison to [ 177 Lu]Lu-CHX-A″-DTPA using a TSKgel SuperSW mAb HR column as described under . ( B ) IRF of [ 177 Lu]Lu-CHX-A″-DTPA-7F5. The radiolabeled Ab was incubated with increasing amounts of PC3-P cells at 37 °C for 2 h. Afterwards, the radiolabeled cells were isolated by filtration through a glass fiber filter. The radioactivity bound to the filter was counted in a gamma counter. Totally applied activity was divided by the specifically bound radioactivity on the cells and then plotted as a function of the reciprocal antigen concentration. The Y intercept represents the reciprocal of the IRF (n = 3, value in % ± SEM).

Techniques: Control, Labeling, Filtration, Comparison, Incubation, Isolation, Radioactivity, Activity Assay, Concentration Assay

Stability of [ 177 Lu]Lu-CHX-A″-DTPA-7F5. ( A ) SDS-PAGE of [ 177 Lu]Lu-CHX-A″-DTPA-7F5 two days after radiolabeling. [ 177 Lu]Lu-CHX-A″-DTPA-7F5 was separated under reducing (( a , b ), left lanes) and non-reducing (( a , b ), right lanes) conditions. Proteins were detected by either silver staining ( a ) or autoradiography ( b ). M, molecular weight marker. ( B ) Incubation of [ 177 Lu]Lu-CHX-A″-DTPA-7F5 with PBS, cell culture medium, or human serum for 0, 1, or 2 days. After each time point, the intact amount of radiolabeled Ab was absorbed on a protein A affinity column and measured as described under . The stability represents the portion of protein A bound activity (n = 3, stability (%) ± SD).

Journal: International Journal of Molecular Sciences

Article Title: Preclinical Characterization of the 177 Lu-Labeled Prostate Stem Cell Antigen (PSCA)-Specific Monoclonal Antibody 7F5

doi: 10.3390/ijms24119420

Figure Lengend Snippet: Stability of [ 177 Lu]Lu-CHX-A″-DTPA-7F5. ( A ) SDS-PAGE of [ 177 Lu]Lu-CHX-A″-DTPA-7F5 two days after radiolabeling. [ 177 Lu]Lu-CHX-A″-DTPA-7F5 was separated under reducing (( a , b ), left lanes) and non-reducing (( a , b ), right lanes) conditions. Proteins were detected by either silver staining ( a ) or autoradiography ( b ). M, molecular weight marker. ( B ) Incubation of [ 177 Lu]Lu-CHX-A″-DTPA-7F5 with PBS, cell culture medium, or human serum for 0, 1, or 2 days. After each time point, the intact amount of radiolabeled Ab was absorbed on a protein A affinity column and measured as described under . The stability represents the portion of protein A bound activity (n = 3, stability (%) ± SD).

Techniques: SDS Page, Radioactivity, Silver Staining, Autoradiography, Molecular Weight, Marker, Incubation, Cell Culture, Affinity Column, Activity Assay

( A ) Time-dependent PSCA-mediated uptake of [ 177 Lu]Lu-CHX-A″-DTPA-7F5 by PC3-P cells. The samples were incubated at 37 °C; at indicated time points, the amount of cell-associated and incorporated radioactivity was determined. ( B ) Representative graph of a saturation binding assay with [ 177 Lu]Lu-DTPA-7F5 on intact PC3-P cells. PC3-P cells were incubated for 2 h at 37 °C with increasing [ 177 Lu]Lu-CHX-A″-DTPA-7F5 concentrations (n = 3). Nonspecific binding was determined in the presence of 0.5 µM non-labeled anti-PSCA mAb 7F5. Red is the fitted line after subtracting the values of the non-specific binding from those of the total binding.

Journal: International Journal of Molecular Sciences

Article Title: Preclinical Characterization of the 177 Lu-Labeled Prostate Stem Cell Antigen (PSCA)-Specific Monoclonal Antibody 7F5

doi: 10.3390/ijms24119420

Figure Lengend Snippet: ( A ) Time-dependent PSCA-mediated uptake of [ 177 Lu]Lu-CHX-A″-DTPA-7F5 by PC3-P cells. The samples were incubated at 37 °C; at indicated time points, the amount of cell-associated and incorporated radioactivity was determined. ( B ) Representative graph of a saturation binding assay with [ 177 Lu]Lu-DTPA-7F5 on intact PC3-P cells. PC3-P cells were incubated for 2 h at 37 °C with increasing [ 177 Lu]Lu-CHX-A″-DTPA-7F5 concentrations (n = 3). Nonspecific binding was determined in the presence of 0.5 µM non-labeled anti-PSCA mAb 7F5. Red is the fitted line after subtracting the values of the non-specific binding from those of the total binding.

Techniques: Incubation, Radioactivity, Saturation Assay, Binding Assay, Labeling

Biodistribution of [ 177 Lu]Lu-CHX-A″-DTPA-7F5 in tumor and normal tissues at 1 h and 48 h post injection. [ 177 Lu]Lu-CHX-A″-DTPA-7F5 was injected in male PC3-P tumor-bearing mice (n = 4). ( A ) After 1 h and 48 h, SUVs in organs and tissues were determined (mean ± SEM). ( B ) Accumulation of [ 177 Lu]Lu-CHX-A″-DTPA-7F5 determined as sum of liver and intestine as well as of kidney and urine. ( C ) SUV ratios of tumor-to-muscle and tumor-to-blood. WAT—white adipose tissue; BAT—brown adipose tissue; t -test: * p < 0.05; ** p < 0.01; *** p < 0.001.

Journal: International Journal of Molecular Sciences

Article Title: Preclinical Characterization of the 177 Lu-Labeled Prostate Stem Cell Antigen (PSCA)-Specific Monoclonal Antibody 7F5

doi: 10.3390/ijms24119420

Figure Lengend Snippet: Biodistribution of [ 177 Lu]Lu-CHX-A″-DTPA-7F5 in tumor and normal tissues at 1 h and 48 h post injection. [ 177 Lu]Lu-CHX-A″-DTPA-7F5 was injected in male PC3-P tumor-bearing mice (n = 4). ( A ) After 1 h and 48 h, SUVs in organs and tissues were determined (mean ± SEM). ( B ) Accumulation of [ 177 Lu]Lu-CHX-A″-DTPA-7F5 determined as sum of liver and intestine as well as of kidney and urine. ( C ) SUV ratios of tumor-to-muscle and tumor-to-blood. WAT—white adipose tissue; BAT—brown adipose tissue; t -test: * p < 0.05; ** p < 0.01; *** p < 0.001.

Techniques: Injection

SPECT/CT imaging of [ 177 Lu]Lu-CHX-A″-DTPA-7F5 in PC3-P and PC3 tumor-bearing mice between 1 h and 144 h p.i. [ 177 Lu]Lu-CHX-A″-DTPA-7F5 was injected in female mice bearing both PC3 (left flank) and PC3-P tumor (right flank) (n = 2). ( A ) Maximum intensity projection (MIP) of the [ 177 Lu]Lu-CHX-A″-DTPA-7F5 distribution in a representative tumor xenograft -bearing mouse at 1, 6, 24, 48 and 144 h p.i. H—heart, L—liver, S—spleen, PC3—PC3 tumor, PC3-P—PC3-P tumor, M—potential metastasis. ( B ) Sagital (s), transaxial (t), and coronal (c) sections of two potential metastases near the spinal cord (inside of white box) at 144 h p.i. ( C ) SUV estimated from SPECT images for heart (blood), liver, and spleen, as well as PC-3 and PC3-P tumor at 1, 6, 24, 48, and 144 h p.i. (mean ± SD, n = 2). ( D ) SUV ratios of PC3-P-to-blood and PC3-P-to-PC3 at 1, 6, 24, 48, and 144 h p.i. (mean ± SD, n = 2).

Journal: International Journal of Molecular Sciences

Article Title: Preclinical Characterization of the 177 Lu-Labeled Prostate Stem Cell Antigen (PSCA)-Specific Monoclonal Antibody 7F5

doi: 10.3390/ijms24119420

Figure Lengend Snippet: SPECT/CT imaging of [ 177 Lu]Lu-CHX-A″-DTPA-7F5 in PC3-P and PC3 tumor-bearing mice between 1 h and 144 h p.i. [ 177 Lu]Lu-CHX-A″-DTPA-7F5 was injected in female mice bearing both PC3 (left flank) and PC3-P tumor (right flank) (n = 2). ( A ) Maximum intensity projection (MIP) of the [ 177 Lu]Lu-CHX-A″-DTPA-7F5 distribution in a representative tumor xenograft -bearing mouse at 1, 6, 24, 48 and 144 h p.i. H—heart, L—liver, S—spleen, PC3—PC3 tumor, PC3-P—PC3-P tumor, M—potential metastasis. ( B ) Sagital (s), transaxial (t), and coronal (c) sections of two potential metastases near the spinal cord (inside of white box) at 144 h p.i. ( C ) SUV estimated from SPECT images for heart (blood), liver, and spleen, as well as PC-3 and PC3-P tumor at 1, 6, 24, 48, and 144 h p.i. (mean ± SD, n = 2). ( D ) SUV ratios of PC3-P-to-blood and PC3-P-to-PC3 at 1, 6, 24, 48, and 144 h p.i. (mean ± SD, n = 2).

Techniques: Single Photon Emission Computed Tomography, Imaging, Injection

7f5 mab Search Results

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